Development of a Biosensor for Electrochemical Detection of Tumor-Associated Proteins in Blood Plasma of Cancer Patients by Aptamers

Zamay, G. S.; Zamay, T. N.; Kolovskaya, O. S.; Krat, A. V.; Glazirin, Yu. E.; Dubinina, A. V.; Zamay, A. S.

doi:10.1134/S1607672916010208

Автор:

Zamay, G. S.

Zamay, T. N.

Kolovskaya, O. S.

Krat, A. V.

Glazirin, Yu. E.

Dubinina, A. V.

Zamay, A. S.

Коллективный автор:

Институт фундаментальной биологии и биотехнологии

Кафедра медицинской биологии

Дата:

2016

Журнал:

Doklady Biochemistry and Biophysics

Квартиль журнала в Scopus:

Квартиль журнала в Web of Science:

Библиографическое описание:

Zamay, G. S. Development of a Biosensor for Electrochemical Detection of Tumor-Associated Proteins in Blood Plasma of Cancer Patients by Aptamers [Текст] / G. S. Zamay, T. N. Zamay, O. S. Kolovskaya, A. V. Krat, Yu. E. Glazirin, A. V. Dubinina, A. S. Zamay // Doklady Biochemistry and Biophysics. — 2016. — Т. 466 (№ 6). — С. 70-73

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.

Аннотация:

A molecular biosensor based on DNA aptamers (aptasensors) for the diagnosis of lung cancer in blood plasma samples was designed. To create the aptasensor, aptamer 17_80, obtained in the study of postoperative material, was used. The affinity and binding selectivity of the aptamer 17_80 to the lung tumor tissue was confirmed on histological sections of postmortem samples of lung tissue. Using affinity enrichment and mass spectrometry, a possible target molecular of the aptamer 17_80, vimentin, was found.

Коллекции:

Статьи в научных журналах (эффективный контракт) [5211]

Метаданные:

Показать полную информацию

Показаны похожие ресурсы по названию, автору или тематике.

Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples

Zamay, G. S.; Zamay, T. N.; Kolovskii, V. A.; Shabanov, A. V.; Glazirin, Yu. E.; Veprintsev, D. V.; Krat, A. V.; Zamay, S. S.; Kolovskaya, O. S.; Gargaun, A. E.; Sokolov, A. A.; Modestov, A. A.; Artyukhov, I. P.; Chesnokov, N. V.; Petrova, M. M.; Berezovski, M. V.; Zamay, A. S. (2016-09)

The development of an aptamer-based electrochemical sensor for lung cancer detection is presented in the work.A highly specific DNA-aptamer, LC-18, selected to postoperative lung cancer tissues was immobilized onto a gold ...
The antitumor Effect of Magnetic Nanodiscs and Aptamer Conjugates

Kim, P. D.; Zamay, S. S.; Zamay, T. N.; Prokopenko, V. S.; Kolovskaya, O. S.; Zamay, G. S.; Princ, V. Ya.; Seleznev, V. A.; Komonov, A. I.; Spivak, E. A.; Rudenko, R. Yu.; Dubinina, A. V.; Komarov, A. V.; Denisenko, V. V.; Komarova, M. A.; Sokolov, A. E.; Narodov, A. A.; Zjivaev, V. P.; Zamay, A. S. (2016)

Here we descrabe a method of forming large arrays of free magnetic Ni-nanodiscs 50 nm thick coated on both sides with layers of 5 nm thick Au. The antitumor effect of the magnetic nickel gold-cjated nanodiscs and DNA aptamer ...
The antitumor effect of magnetic nanodiscs and DNA aptamer conjugates

Kim, P. D.; Zamay, S. S.; Zamay, T. N.; Procopenko, V. S.; Kolovskaya, O. S.; Zamay, G. S.; Princ, V. Y.; Seleznev, V. A.; Komorov, A. I.; Spivak, E. A.; Rudenko, R. Y.; Dybinina, A. V.; Komarov, A. V.; Denisenko, V. V.; Komarova, M. A.; Sokolov, A. E.; Narodov, A. A.; Zjivaev, V. P.; Zamay, A. S. (2016)

Here we describe a method of forming large arrays (up to 109 pieces) of free magnetic Ni nano disks 50 nm thick coated on both sides with layers of 5 nm thick Au. The antitumor effect of the magnetic nickel gold coated ...
Selection of DNA Aptamers for Breast Cancer

Zamay, G. S.; Belyanina, I. V.; Zamay, A. S.; Komarova, M. A.; Krat, A. V.; Eremina, E. N.; Zukov, R. A.; Sokolov, A. E.; Zamay, T. N. (2016)

A method of selection of DNA aptamers to breast tumor tissue based on the use of potoperative material has been developed. Breast cancer tissue were used as the positive target; the negative targets included benign tumor ...
In Vivo Cancer Cells Elimination Guided by Aptamer-Functionalized Gold-Coated Magnetic Nanoparticles and Controlled with Low Frequency Alternating Magnetic Field

Irina V. Belyanina; Tatiana N. Zamay; Galina S. Zamay; Sergey S. Zamay; Olga S. Kolovskaya; Tatiana I. Ivanchenko; Valery V. Denisenko; Andrey K. Kirichenko; Yury E. Glazyrin; Irina V. Garanzha; Valentina V. Grigorieva; Alexandr V. Shabanov; Dmitry V. Veprintsev; Alexey E. Sokolov; Vladimir M. Sadovskii; Ana, Gargaun; Maxim V. Berezovski; Anna S. Kichkailo (2017-07)

Biomedical applications of magnetic nanoparticles in a magnetic field have exceeded many expectations in cancer therapy. Magnetic nanoparticles are effective heat mediators, drug nanocarriers, and contrast agents; various ...