Development of a Biosensor for Electrochemical Detection of Tumor-Associated Proteins in Blood Plasma of Cancer Patients by Aptamers

Abstract

A molecular biosensor based on DNA aptamers (aptasensors) for the diagnosis of lung cancer in blood plasma samples was designed. To create the aptasensor, aptamer 17_80, obtained in the study of postoperative material, was used. The affinity and binding selectivity of the aptamer 17_80 to the lung tumor tissue was confirmed on histological sections of postmortem samples of lung tissue. Using affinity enrichment and mass spectrometry, a possible target molecular of the aptamer 17_80, vimentin, was found.