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Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoatemonomer by Cupriavidus eutrophus B10646
Автор | Volova, T. G. | |
Автор | Syrvacheva, D. A. | |
Автор | Zhila, N. O. | |
Автор | Sukovatiy, A. G. | |
Дата внесения | 2016-11-11T08:49:13Z | |
Дата, когда ресурс стал доступен | 2016-11-11T08:49:13Z | |
Дата публикации | 2016 | |
Библиографическое описание | Volova, T. G. Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoatemonomer by Cupriavidus eutrophus B10646 [Текст] / T. G. Volova, D. A. Syrvacheva, N. O. Zhila, A. G. Sukovatiy // Journal of Chemical Technology and Biotechnology. — 2016. — Т. 91 (№ 2). — С. 416-425 | |
ISSN | 02682575 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/27831 | |
Аннотация | BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). Themost commonapproach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this studywas to optimize the process of production of P(3HB-co-3HHx) copolymers containing highmolar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5–6 g L-1) and high content of the polymer (60–75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. | |
Ссылка на другой сайт | http://onlinelibrary.wiley.com/doi/10.1002/jctb.4592/abstract | |
Тема | wild-type strain Cupriavidus eutrophus B10646 | |
Тема | growth kinetics | |
Тема | poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) | |
Тема | physicochemical and mechanical properties | |
Название | Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoatemonomer by Cupriavidus eutrophus B10646 | |
Тип | Journal Article | |
Тип | Journal Article Preprint | |
Страницы | 416-425 | |
ГРНТИ | 34.27 | |
Дата обновления | 2016-11-11T08:49:13Z | |
DOI | 10.1002/jctb.4592 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Базовая кафедра биотехнологии | |
Журнал | Journal of Chemical Technology and Biotechnology | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q1 |