Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoatemonomer by Cupriavidus eutrophus B10646
URI (for links/citations):http://elib.sfu-kras.ru/handle/2311/27831
Volova, T. G.
Syrvacheva, D. A.
Zhila, N. O.
Sukovatiy, A. G.
Институт фундаментальной биологии и биотехнологии
Базовая кафедра биотехнологии
Journal Name:Journal of Chemical Technology and Biotechnology
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q1
Bibliographic Citation:Volova, T. G. Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoatemonomer by Cupriavidus eutrophus B10646 [Текст] / T. G. Volova, D. A. Syrvacheva, N. O. Zhila, A. G. Sukovatiy // Journal of Chemical Technology and Biotechnology. — 2016. — Т. 91 (№ 2). — С. 416-425
BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). Themost commonapproach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this studywas to optimize the process of production of P(3HB-co-3HHx) copolymers containing highmolar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5–6 g L-1) and high content of the polymer (60–75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers.