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Tatiana, Volova
Aleksey, Demidenko
Evgeniy, Kiselev
Sergey, Baranovskiy
Ekaterina, Shishatskaya
Natalia, Zhila
2020-01-20T07:56:50Z
2020-01-20T07:56:50Z
2019
Tatiana, Volova. Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production [Текст] / Volova Tatiana, Demidenko Aleksey, Kiselev Evgeniy, Baranovskiy Sergey, Shishatskaya Ekaterina, Zhila Natalia // Applied Microbiology and Biotechnology. — 2019. — Т. 103 (№ 1). — С. 225-237
01757598
https://link.springer.com/article/10.1007/s00253-018-9460-0
http://elib.sfu-kras.ru/handle/2311/129289
The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.
Glycerol
Polyhydroxyalkanoates
Synthesis
Productivity
Properties
Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production
Journal Article
Journal Article Preprint
225-237
62.09
2020-01-20T07:56:50Z
10.1007/s00253-018-9460-0
Институт фундаментальной биологии и биотехнологии
Базовая кафедра биотехнологии
Applied Microbiology and Biotechnology
Q1
Q2


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