Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production
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https://link.springer.com/article/10.1007/s00253-018-9460-0https://elib.sfu-kras.ru/handle/2311/129289
Автор:
Tatiana, Volova
Aleksey, Demidenko
Evgeniy, Kiselev
Sergey, Baranovskiy
Ekaterina, Shishatskaya
Natalia, Zhila
Коллективный автор:
Институт фундаментальной биологии и биотехнологии
Базовая кафедра биотехнологии
Дата:
2019Журнал:
Applied Microbiology and BiotechnologyКвартиль журнала в Scopus:
Q1Квартиль журнала в Web of Science:
Q2Библиографическое описание:
Tatiana, Volova. Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production [Текст] / Volova Tatiana, Demidenko Aleksey, Kiselev Evgeniy, Baranovskiy Sergey, Shishatskaya Ekaterina, Zhila Natalia // Applied Microbiology and Biotechnology. — 2019. — Т. 103 (№ 1). — С. 225-237Аннотация:
The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.