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Shining light on the secreted luciferases of marine copepods: current knowledge and applications
Автор | Markova, S. V. | |
Автор | Larionova, M. D. | |
Автор | Vysotski, E. S. | |
Дата внесения | 2020-01-20T07:16:28Z | |
Дата, когда ресурс стал доступен | 2020-01-20T07:16:28Z | |
Дата публикации | 2019-05 | |
Библиографическое описание | Markova, S. V. Shining light on the secreted luciferases of marine copepods: current knowledge and applications [Текст] / S. V. Markova, M. D. Larionova, E. S. Vysotski // Photochemistry and Photobiology. — 2019. — Т. 95 (№ 3). — С. 705-721 | |
ISSN | 00318655 | |
URI (для ссылок/цитирований) | https://onlinelibrary.wiley.com/doi/10.1111/php.13077 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/128873 | |
Описание | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. | |
Аннотация | Copepod luciferases -a family of small secretory proteins of 18.4-24.3 kDa, including a signal peptide-are responsible for bright secreted bioluminescence of some marine copepods. The copepod luciferases use coelenterazine as a substrate to produce blue light in a simple oxidation reaction without any additional cofactors. They do not share sequence or structural similarity with other identified bioluminescent proteins including coelenterazine-dependent Renilla and Oplophorus luciferases. The small size, strong luminescence activity and high stability, including thermostability, make secreted copepod luciferases very attractive candidates as reporter proteins which are particularly useful for nondisruptive reporter assays and for high-throughput format. The most known and extensively investigated representatives of this family are the first cloned GpLuc and MLuc luciferases from copepods Gaussia princeps and Metridia longa, respectively. Immediately after cloning, these homologous luciferases were successfully applied as bioluminescent reporters in vivo and in vitro, and since then, the scope of their applications continues to grow. This review is an attempt to systemize and critically evaluate the data scattered through numerous articles regarding the main structural features of copepod luciferases, their luminescent and physicochemical properties. We also review the main trends of their application as bioluminescent reporters in cell and molecular biology. | |
Тема | bioluminescence | |
Тема | coelenterazine | |
Тема | secreted luciferase | |
Тема | bioluminescent reporter | |
Тема | thermostability | |
Тема | secreted reporter assay | |
Название | Shining light on the secreted luciferases of marine copepods: current knowledge and applications | |
Тип | Journal Article | |
Тип | Journal Article Postprint | |
Страницы | 705-721 | |
ГРНТИ | 34.17.15 | |
Дата обновления | 2020-01-20T07:16:27Z | |
DOI | 10.1111/php.13077 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Базовая кафедра биотехнологии | |
Журнал | Photochemistry and Photobiology | |
Квартиль журнала в Scopus | Q2 | |
Квартиль журнала в Web of Science | Q3 |