Shining light on the secreted luciferases of marine copepods: current knowledge and applications
URI (for links/citations):https://onlinelibrary.wiley.com/doi/10.1111/php.13077
Markova, S. V.
Larionova, M. D.
Vysotski, E. S.
Институт фундаментальной биологии и биотехнологии
Базовая кафедра биотехнологии
Journal Name:Photochemistry and Photobiology
Journal Quartile in Scopus:Q2
Journal Quartile in Web of Science:Q3
Bibliographic Citation:Markova, S. V. Shining light on the secreted luciferases of marine copepods: current knowledge and applications [Текст] / S. V. Markova, M. D. Larionova, E. S. Vysotski // Photochemistry and Photobiology. — 2019. — Т. 95 (№ 3). — С. 705-721
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
Copepod luciferases -a family of small secretory proteins of 18.4-24.3 kDa, including a signal peptide-are responsible for bright secreted bioluminescence of some marine copepods. The copepod luciferases use coelenterazine as a substrate to produce blue light in a simple oxidation reaction without any additional cofactors. They do not share sequence or structural similarity with other identified bioluminescent proteins including coelenterazine-dependent Renilla and Oplophorus luciferases. The small size, strong luminescence activity and high stability, including thermostability, make secreted copepod luciferases very attractive candidates as reporter proteins which are particularly useful for nondisruptive reporter assays and for high-throughput format. The most known and extensively investigated representatives of this family are the first cloned GpLuc and MLuc luciferases from copepods Gaussia princeps and Metridia longa, respectively. Immediately after cloning, these homologous luciferases were successfully applied as bioluminescent reporters in vivo and in vitro, and since then, the scope of their applications continues to grow. This review is an attempt to systemize and critically evaluate the data scattered through numerous articles regarding the main structural features of copepod luciferases, their luminescent and physicochemical properties. We also review the main trends of their application as bioluminescent reporters in cell and molecular biology.