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Wu, Yu-Ting
Yang, Yu-Cheng
Chiang, Pei-Wen
Tseng, Ching-Hung
Chiu, Hsiu-Hui
Saeed, Isaam
Baatar, Bayanmunkh
Рогозин, Денис
Halgamuge, Saman
Degermendzhi, Andrei
Tang, Sen-Lin
2019-07-01T07:28:40Z
2019-07-01T07:28:40Z
2018-08
Wu, Yu-Ting. Comprehensive Insights into Composition, Metabolic Potentials, and Interactions among Archaeal, Bacterial, and Viral Assemblages in Meromictic Lake Shunet in Siberia [Текст] / Yu-Ting Wu, Yu-Cheng Yang, Pei-Wen Chiang, Ching-Hung Tseng, Hsiu-Hui Chiu, Isaam Saeed, Bayanmunkh Baatar, Денис Рогозин, Saman Halgamuge, Andrei Degermendzhi, Sen-Lin Tang // Frontiers in Microbiology: Aquatic Microbiology. — 2018. — Т. 9 (№ 1763). — С. 1763
1664302X
http://journal.frontiersin.org/article/10.3389/fmicb.2018.01763/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Microbiology&id=366441
http://elib.sfu-kras.ru/handle/2311/111205
Microorganisms are critical to maintaining stratified biogeochemical characteristics in meromictic lakes; however, their community composition and potential roles in nutrient cycling are not thoroughly described. Both metagenomics and metaviromics were used to determine the composition and capacity of archaea, bacteria, and viruses along the water column in the landlocked meromictic Lake Shunet in Siberia. Deep sequencing of 265 Gb and high-quality assembly revealed a near-complete genome corresponding to Nonlabens sp. sh3vir. in a viral sample and 38 bacterial bins (0.2–5.3Mb each). The mixolimnion (3.0m) had the most diverse archaeal, bacterial, and viral communities, followed by the monimolimnion (5.5m) and chemocline (5.0m). The bacterial and archaeal communities were dominated by Thiocapsa and Methanococcoides, respectively, whereas the viral community was dominated by Siphoviridae. The archaeal and bacterial assemblages and the associated energy metabolism were significantly related to the various depths, in accordance with the stratification of physicochemical parameters. Reconstructed elemental nutrient cycles of the three layers were interconnected, including co-occurrence of denitrification and nitrogen fixation in each layer and involved unique processes due to specific biogeochemical properties at the respective depths. According to the gene annotation, several pre-dominant yet unknown and uncultured bacteria also play potentially important roles in nutrient cycling. Reciprocal BLAST analysis revealed that the viruses were specific to the host archaea and bacteria in the mixolimnion. This study provides insights into the bacterial, archaeal, and viral assemblages and the corresponding capacity potentials in Lake Shunet, one of the three meromictic lakes in central Asia. Lake Shunet was determined to harbor specific and diverse viral, bacterial, and archaeal communities that intimately interacted, revealing patterns shaped by indigenous physicochemical parameters.
Lake Shunet
metagenomics
archaeal
bacterial and viral assemblages
meromictic lake
Comprehensive Insights into Composition, Metabolic Potentials, and Interactions among Archaeal, Bacterial, and Viral Assemblages in Meromictic Lake Shunet in Siberia
Journal Article
Journal Article Preprint
1763
34.27.23
2019-07-01T07:28:40Z
10.3389/fmicb.2018.01763
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Frontiers in Microbiology
Q1
Q1


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