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Волова, Т. Г.
Меньшикова, О. Н.
Жила, Н. О.
Васильев, А. Д.
Киселев, Е. Г.
Петерсон, И. В.
Шишацкая, Е. И.
Sabu, Thomas
2019-07-01T07:27:22Z
2019-07-01T07:27:22Z
2018-07
Волова, Т. Г. Biosynthesis and properties of P(3HB‐co‐3HV‐co‐3H4MV) produced by using the wild‐type strain Cupriavidus eutrophus B‐10646 [Текст] / Т. Г. Волова, О. Н. Меньшикова, Н. О. Жила, А. Д. Васильев, Е. Г. Киселев, И. В. Петерсон, Е. И. Шишацкая, Thomas Sabu // Journal of Chemical Technology and Biotechnology. — 2018.
02682575
https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.5763
http://elib.sfu-kras.ru/handle/2311/110939
BACKGROUND Polyhydroxyalkanoates (PHA) containing 3‐hydroxy‐4‐methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate‐co‐3‐hydroxy‐4‐methylvalerate) [P(3HB‐co‐3 HV‐co‐3H4MV)] by Cupriavidus eutrophus B‐10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS It was shown that 4‐methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L−1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry
polyhydroxyalkanoates
3-hydroxy-4-methylvalerate
synthesis
physicochemical properties
Biosynthesis and properties of P(3HB‐co‐3HV‐co‐3H4MV) produced by using the wild‐type strain Cupriavidus eutrophus B‐10646
Journal Article
Journal Article Preprint
34.27.19
2019-07-01T07:27:22Z
10.1002/jctb.5763
Институт фундаментальной биологии и биотехнологии
Институт инженерной физики и радиоэлектроники
Кафедра медицинской биологии
Базовая кафедра физики твердого тела и нанотехнологий
Базовая кафедра биотехнологии
Journal of Chemical Technology and Biotechnology
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