Biosynthesis and properties of P(3HB‐co‐3HV‐co‐3H4MV) produced by using the wild‐type strain Cupriavidus eutrophus B‐10646
URI (for links/citations):https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.5763
Волова, Т. Г.
Меньшикова, О. Н.
Жила, Н. О.
Васильев, А. Д.
Киселев, Е. Г.
Петерсон, И. В.
Шишацкая, Е. И.
Институт фундаментальной биологии и биотехнологии
Институт инженерной физики и радиоэлектроники
Кафедра медицинской биологии
Базовая кафедра физики твердого тела и нанотехнологий
Базовая кафедра биотехнологии
Journal Name:Journal of Chemical Technology and Biotechnology
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q1
Bibliographic Citation:Волова, Т. Г. Biosynthesis and properties of P(3HB‐co‐3HV‐co‐3H4MV) produced by using the wild‐type strain Cupriavidus eutrophus B‐10646 [Текст] / Т. Г. Волова, О. Н. Меньшикова, Н. О. Жила, А. Д. Васильев, Е. Г. Киселев, И. В. Петерсон, Е. И. Шишацкая, Thomas Sabu // Journal of Chemical Technology and Biotechnology. — 2018.
BACKGROUND Polyhydroxyalkanoates (PHA) containing 3‐hydroxy‐4‐methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate‐co‐3‐hydroxy‐4‐methylvalerate) [P(3HB‐co‐3 HV‐co‐3H4MV)] by Cupriavidus eutrophus B‐10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS It was shown that 4‐methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L−1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry