Applying methods of hard tissues preparation for wood anatomy: Imaging polished samples embedded in polymethylmethacrylate
URI (для ссылок/цитирований):
https://www.sciencedirect.com/science/article/pii/S1125786518301073https://elib.sfu-kras.ru/handle/2311/110553
Автор:
Arzac, Alberto
López-Cepero, José M
Babushkina, Elena
Gomez, Santiago
Коллективный автор:
Институт экологии и географии
Хакасский технический институт — филиал СФУ
Кафедра экологии и природопользования
Кафедра строительства
Дата:
2018-10Журнал:
DendrochronologiaКвартиль журнала в Scopus:
Q1Квартиль журнала в Web of Science:
Q1Библиографическое описание:
Arzac, Alberto. Applying methods of hard tissues preparation for wood anatomy: Imaging polished samples embedded in polymethylmethacrylate [Текст] / Alberto Arzac, José M López-Cepero, Elena Babushkina, Santiago Gomez // Dendrochronologia. — 2018. — С. 76-81Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
Аннотация:
Cambial activity records short and long-term environmental signals in xylem anatomy, creating a permanent
archive. Quantitative wood anatomy deciphers the relationship between cell structure and function in a spatiotemporal
context. Obtaining high-resolution images of wood anatomical preparations is a critical stage in the
process of decoding this information. Damage to cellular structures when sectioning by microtome is one of the
main problems in the preparation of high-quality micro-sections. Cell damage leads to the occurrence of artifacts
– most often related to broken cell walls – hindering the performance of image recognition programs, and
increasing the time spent on the manual editing of images. In this work, we propose an alternative method to
microtomy, based on embedding-polishing protocols established for hard tissue preparation. Wood samples are
embedded in a transparent and non-reactive resin as polymethylmethacrylate (PMM) that is subsequently
ground and polished. Being able to acquire images from the stained or unstained polished surfaces of the PMMblocks
and sections (thinner than 100 μm) by using a wide range of optical methods such as reflected polarizing
microscopy, epifluorescence microscopy, bright-field microscopy with diffuse illumination and circularly polarizing
microscopy. This embedding method improves the mechanical integrity and quality of wood anatomical
preparations, eliminating the problem of broken cell walls. Furthermore, this technique allows the preparation
and analysis of large tissue surfaces.