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Alieva, R. R.
Tomilin, F. C.
Kuzubov, A. A.
Ovchinnikov, S. G.
Kudryasheva, N. S.
2016-11-11T08:52:14Z
2016-11-11T08:52:14Z
2016
Alieva, R. R. Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study. [Текст] / R. R. Alieva, F. C. Tomilin, A. A. Kuzubov, S. G. Ovchinnikov, N. S. Kudryasheva // Journal of Photochemistry and Photobiology B: Biology. — 2016. — Т. 162. — С. 318-323
10111344
https://elib.sfu-kras.ru/handle/2311/27990
Coelenteramide-containing fluorescent proteins are products of bioluminescent reactions of marine coelenterates. They are called ‘discharged photoproteins’. Their light-induced fluorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-induced fluorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexcitation to the upper electron-excited states (260-300 nm) of the discharged photoproteins initiates a fluorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UV fluorescence, the light-induced fluorescence spectra of coelenteramide (CLM), fluorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330 nm) in the near UV region was observed in CLM fluorescence at higher excitation energy (260-300 nm). Quantum chemical calculations by time depending method with B3LYP / cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UV fluorescence peak. Quantum yields of CLM fluorescence in methanol were 0.028 ± 0.005 at 270-340 nm photoexcitation. A conclusion was made that the UV emission of CLM might contribute to the UV fluorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures – complexes of proteins with low-weight aromatic molecules.
http://www.sciencedirect.com/science/article/pii/S1011134416304262
coelenteramide
fluorescent protein
discharged photoproteins
obelin
aequorin
fluorescence
excitation energy
B3LYP
Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study.
Journal Article
Journal Article Postprint
318-323
34.17.09
2016-11-11T08:52:14Z
10.1016/j.jphotobiol.2016.07.004
Научно-исследовательская часть
Journal of Photochemistry and Photobiology B: Biology
Q2


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