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Transferability and polymorphism of SSR markers located in flavonoid pathway genes in fragaria and rubus species
Автор | Lebedev Vadim G. | |
Автор | Subbotina Natalya M. | |
Автор | Maluchenko Oleg P. | |
Автор | Lebedeva Tatyana N. | |
Автор | Krutovsky Konstantin V. | |
Автор | Shestibratov Konstantin A. | |
Дата внесения | 2021-02-15T12:36:46Z | |
Дата, когда ресурс стал доступен | 2021-02-15T12:36:46Z | |
Дата публикации | 2020 | |
ISSN | 20734425 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/137960 | |
Описание | Статья из журнала. | |
Аннотация | Strawberry (Fragaria) and raspberry (Rubus) are very popular crops, and improving their nutritional quality and disease resistance are important tasks in their breeding programs that are becoming increasingly based on use of functional DNA markers. We identified 118 microsatellite (simple sequence repeat—SSR) loci in the nucleotide sequences of flavonoid biosynthesis and pathogenesis-related genes and developed 24 SSR markers representing some of these structural and regulatory genes. These markers were used to assess the genetic diversity of 48 Fragaria and Rubus specimens, including wild species and rare cultivars, which differ in berry color, ploidy, and origin. We have demonstrated that a high proportion of the developed markers are transferable within and between Fragaria and Rubus genera and are polymorphic. Transferability and polymorphism of the SSR markers depended on location of their polymerase chain reaction (PCR) primer annealing sites and microsatellite loci in genes, respectively. High polymorphism of the SSR markers in regulatory flavonoid biosynthesis genes suggests their allelic variability that can be potentially associated with differences in flavonoid accumulation and composition. This set of SSR markers may be a useful molecular tool in strawberry and raspberry breeding programs for improvement anthocyanin related traits. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. | |
Издатель | MDPI AG | |
Является частью серии | Vol. 11, Is. 1 | |
Ссылка на другой сайт | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077192119&doi=10.3390%2fgenes11010011&partnerID=40&md5=397782ac4bfae1ab5aa0d6c0fff38cfc | |
Ссылка на другой сайт | http://apps.webofknowledge.com/InboundService.do?SID=X1HogM8pCtTkhgkuprQ&product=WOS&UT=WOS%3A000514898000005&SrcApp=Alerting&DestFail=http%3A%2F%2Fwww.webofknowledge.com&Init=Yes&action=retrieve&SrcAuth=Alerting&Func=Frame&customersID=Alerting&IsProductCode=Yes&mode=FullRecord | |
Тема | Chitinase | |
Тема | Exons | |
Тема | Flavonoid biosynthesis pathway | |
Тема | Fragaria | |
Тема | Introns | |
Тема | Microsatellites | |
Тема | Polymorphism | |
Тема | Rubus | |
Тема | Transcription factor genes | |
Тема | Transferability | |
Название | Transferability and polymorphism of SSR markers located in flavonoid pathway genes in fragaria and rubus species | |
Тип | Journal Article | |
Контакты автора | Lebedev Vadim G.: Pushchino State Inst Nat Sci, Prospekt Nauki 3, Pushchino 142290, Russia; Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Prospekt Nauki 6, Pushchino 142290, Russia | |
Контакты автора | Subbotina Natalya M.: Pushchino State Inst Nat Sci, Prospekt Nauki 3, Pushchino 142290, Russia; Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Prospekt Nauki 6, Pushchino 142290, Russia | |
Контакты автора | Maluchenko Oleg P.: All Russian Res Inst Agr Biotechnol, Timiriazevskaya Str 42, Moscow 127550, Russia | |
Контакты автора | Lebedeva Tatyana N.: Russian Acad Sci, Inst Physicochem & Biol Problems Soil Sci, Inst Skaya Str 2, Pushchino 142290, Russia | |
Контакты автора | Krutovsky Konstantin V.: Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany; Georg August Univ Gottingen, Ctr Integrated Breeding Res, Albrecht Thaer Weg 3, D-37075 Gottingen, Germany; Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Gubkin Str 3, Moscow 119333, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia | |
Контакты автора | Shestibratov Konstantin A.: Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Prospekt Nauki 6, Pushchino 142290, Russia | |
Страницы | 11 | |
DOI | 10.3390/genes11010011 | |
Журнал | Genes | |
Квартиль журнала в Scopus | да | |
Квартиль журнала в Web of Science | да | |
Шифр в ИРБИС | 701574010-713183021 |