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Functional divergence between evolutionary related LuxG and Fre oxidoreductases of luminous bacteria
Автор | Anna A. Deeva | |
Автор | Evgenia A. Zykova | |
Автор | Elena V. Nemtseva | |
Автор | Valentina A. Kratasyuk | |
Дата внесения | 2020-01-20T08:00:54Z | |
Дата, когда ресурс стал доступен | 2020-01-20T08:00:54Z | |
Дата публикации | 2019-04 | |
Библиографическое описание | Anna A. Deeva. Functional divergence between evolutionary related LuxG and Fre oxidoreductases of luminous bacteria [Текст] / Anna A. Deeva, Evgenia A. Zykova, Elena V. Nemtseva, Valentina A. Kratasyuk // Proteins: Structure, Function and Bioinformatics. — 2019. — Т. 87. — С. 1-7 | |
URI (для ссылок/цитирований) | https://onlinelibrary.wiley.com/doi/full/10.1002/prot.25696 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/129502 | |
Аннотация | In luminous bacteria NAD(P)H:flavin-oxidoreductases LuxG and Fre there are homologous enzymes that could provide a luciferase with reduced flavin. While Fre functions as a housekeeping enzyme, LuxG appears to be a source of reduced flavin for bioluminescence as it is transcribed together with luciferase. This study is aimed at providing the basic conception of Fre and LuxG evolution and revealing the peculiarities of the active site structure resulted from a functional variation within the oxidoreductase family. A phylogenetic analysis has demonstrated that Fre and LuxG oxidoreductases have evolved separately after the gene duplication event, and consequently, they have acquired changes in the conservation of functionally related sites. Namely, different evolutionary rates have been observed at the site responsible for specificity to flavin substrate (Arg 46). Also Tyr 72 forming a part of a mobile loop involved into FAD binding has been found to be conserved among Fre in contrast to LuxG oxidoreductases. The conservation of different amino acid types in NAD(P)H binding site has been defined for Fre (arginine) and LuxG (proline) oxidoreductases. | |
Тема | NAD(P)H:flavin-oxidoreductase | |
Тема | Fre | |
Тема | LuxG | |
Тема | gene duplication | |
Тема | functional divergence | |
Тема | bacterial bioluminescence | |
Название | Functional divergence between evolutionary related LuxG and Fre oxidoreductases of luminous bacteria | |
Тип | Journal Article | |
Тип | Journal Article Preprint | |
Страницы | 1-7 | |
ГРНТИ | 34.15.15 | |
Дата обновления | 2020-01-20T08:00:54Z | |
DOI | 10.1002/prot.25696 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Кафедра биофизики | |
Журнал | Proteins: Structure, Function and Bioinformatics | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q3 |