Triplet Frequencies Implementation in Total Transcriptome Analysis

Abstract

We studied the structuredness in total transcriptome of Siberian larch. To do that, the contigs from total transcriptome has been labeled with the reads comprising the tissue specific transcriptomes, and the distribution of the contigs from the total transcriptome has been developed with respect to the mutual entropy of the frequencies of occurrence of reads from tissue specific transcriptomes. It was found that a number of contigs contain comparable amounts of reads from different tissues, so the chimeric transcripts to be extremely abundant. On the contrary, the transcripts with high tissue specificity do not yield a reliable clustering revealing the tissue specificity. This fact makes usage of total transcriptome for the purposes of differential expression arguable.