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Natalia G.Menzyanova
Svetlana А.Pyatina
Elena D.Nikolaeva
Alexander V.Shabanov
Ivan V.Nemtsev
Dmitry P. Stolyarov
Dmitry B. Dryganov
Eugene V. Sakhnov
Ekaterina I. Shishatskaya
2020-01-20T07:53:51Z
2020-01-20T07:53:51Z
2019-01
Natalia G.Menzyanova. Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients [Текст] / Natalia G.Menzyanova, Svetlana А.Pyatina, Elena D.Nikolaeva, Alexander V.Shabanov, Ivan V.Nemtsev, Dmitry P. Stolyarov, Dmitry B. Dryganov, Eugene V. Sakhnov, Ekaterina I. Shishatskaya // Toxicology Reports. — 2019. — № 6. — С. 74-90
22147500
https://www.sciencedirect.com/science/article/pii/S2214750018306437
https://elib.sfu-kras.ru/handle/2311/129002
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.
Monocytes
Macrophages
Cell morphology
Polyhydroxyalkanoates
Atherosclerosis
Intravascular stenting
Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Journal Article
Published Journal Article
74-90
34.19
2020-01-20T07:53:51Z
10.1016/j.toxrep.2018.11.009
Институт фундаментальной биологии и биотехнологии
Кафедра медицинской биологии
Toxicology Reports
Q2
без квартиля


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