The effect of the ratio of the medium volume and the number of organisms on the results of toxicological experiments

Abstract

Abstract. The dependence of the sensitivity of test organisms of different systematic groups and trophic levels (Escherichia coli bacteria, Chlorella vulgaris microalgae, Daphnia magna crustaceans, Lemna minor aquatic plants) to copper ions on the ratio of the volume of the medium and the number of test objects was studied. A significant decrease in EC50 indices was revealed with an increase in the volume of the test sample per one organism. The possibility of increasing the sensitivity of rapid bioassays on these organisms using the equipment and methodology developed in the Siberian Federal University is shown.