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FLUORESCENT PROTEIN-BASED BIOASSAY FOR LOW-DOSE GAMMA RADIATION
Автор | Petrova, A. S. | |
Автор | Lukonina, A. S. | |
Автор | Dementyev, D. V. | |
Автор | Bolsunovsky, A. Ya. | |
Автор | Popov, A. V. | |
Автор | Кудряшева, Н. С. | |
Дата внесения | 2019-07-01T07:29:50Z | |
Дата, когда ресурс стал доступен | 2019-07-01T07:29:50Z | |
Дата публикации | 2018-07 | |
Библиографическое описание | Petrova, A. S. FLUORESCENT PROTEIN-BASED BIOASSAY FOR LOW-DOSE GAMMA RADIATION [Текст] / A. S. Petrova, A. S. Lukonina, D. V. Dementyev, A. Ya. Bolsunovsky, A. V. Popov, Н. С. Кудряшева // Analytical and bioanalytical Chemistry. — 2018. | |
ISSN | 16182642 | |
URI (для ссылок/цитирований) | https://link.springer.com/article/10.1007/s00216-018-1282-5 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/111446 | |
Описание | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. | |
Аннотация | The study develops an application of fluorescent coelenteramide-containing protein as a color bioassay for radiotoxicity evaluation. Biological effect of chronic low-dose gamma radiation was under investigation. The Cs-137 containing radioactive particle (2.07 mGy/h) was used as a source of low-intensive gamma radiation. ‘Discharged obelin’, product of bioluminescent reaction of marine coelenterate Obelia longissimi, was applied as a representative of the coelenteramide-containing protein group. Coelenteramide, fluorophore of discharged obelin, is a photochemically active molecule; it produces fluorescence forms of different color. Contributions of these forms (‘violet’ and ‘blue-green’) to the visible fluorescence depend on the efficiency of photochemical proton transfer in protein-bound coelenteramide; the contributions are controlled by coelenteramide’ microenvironment, and, hence, evaluate distractive ability and toxicity of gamma radiation. The protein samples were exposed to gamma radiation for 7 and 16 days at 20°C and 5°C respectively; maximal dose accumulated by the samples was 0.35 Gy being close to a tentative limit of a low-dose interval. As a result, two fluorescent characteristics (overall fluorescent intensity and contributions of color components to the fluorescent spectra) are suggested as bioassay parameters. Both parameters demonstrated high sensitivity of the protein-based bioassay to low-dose gamma radiation (to 0.03 -0.05 Gy). Higher temperature (20°C) enhanced the response of the protein to the radiation. The study develops physico-chemical understanding of radiotoxic effects. | |
Тема | fluorescent protein | |
Тема | coelenteramide | |
Тема | discharged photoprotein | |
Тема | multicolor bioassay | |
Тема | radiotoxicity | |
Тема | gamma radiation | |
Название | FLUORESCENT PROTEIN-BASED BIOASSAY FOR LOW-DOSE GAMMA RADIATION | |
Тип | Journal Article | |
Тип | Published Journal Article | |
ГРНТИ | 34.49.19 | |
Дата обновления | 2019-07-01T07:29:50Z | |
DOI | 10.1007/s00216-018-1282-5 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Кафедра биофизики | |
Журнал | Analytical and bioanalytical Chemistry | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q1 |