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Belokon, M. M.
Politov, D. V.
Mudrik, E. A.
Polyakova, T. A.
Shatokhina, A. V.
Belokon, Yu. S.
Oreshkova, N. V.
Putintseva, Yu. A.
Sharov, V. V.
Kuzmin, D. A.
Krutovsky, K. V.
2018-02-07T07:26:39Z
2018-02-07T07:26:39Z
2016-12
Belokon, M. M. Development of Microsatellite Genetic Markers in Siberian Stone Pine (Pinus sibirica Du Tour) Based on the De Novo Whole Genome Sequencing [Текст] / M. M. Belokon, D. V. Politov, E. A. Mudrik, T. A. Polyakova, A. V. Shatokhina, Yu. S. Belokon, N. V. Oreshkova, Yu. A. Putintseva, V. V. Sharov, D. A. Kuzmin, K. V. Krutovsky // Russian Journal of Genetics. — 2016. — Т. 52 (№ 12). — С. 1263-1271
10227954
https://link.springer.com/article/10.1134/S1022795416120036
https://elib.sfu-kras.ru/handle/2311/69653
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
Siberian stone pine, Pinus sibirica Du Tour is one of the most economically and environmentally important forest-forming species of conifers in Russia. To study these forests a large number of highly polymorphic molecular genetic markers, such as microsatellite loci, are required. Prior to the new high-throughput next generation sequencing (NGS) methods, discovery of microsatellite loci and development of micro-satellite markers were very time consuming and laborious. The recently developed draft assembly of the Siberian stone pine genome, sequenced using the NGS methods, allowed us to identify a large number of microsatellite loci in the Siberian stone pine genome and to develop species-specific PCR primers for amplification and genotyping of 70 microsatellite loci. The primers were designed using contigs containing short simple sequence tandem repeats from the Siberian stone pine whole genome draft assembly. Based on the testing of primers for 70 microsatellite loci with tri-, tetra- or pentanucleotide repeats, 18 most promising, reliable and polymorphic loci were selected that can be used further as molecular genetic markers in population genetic studies of Siberian stone pine.
genome microsatellite
markers
whole genome sequencing
Siberian stone pine
genetic diversity
heterozygosity
NGS
Development of Microsatellite Genetic Markers in Siberian Stone Pine (Pinus sibirica Du Tour) Based on the De Novo Whole Genome Sequencing
Journal Article
Journal Article Preprint
1263-1271
34.23
2018-02-07T07:26:38Z
10.1134/S1022795416120036
Институт космических и информационных технологий
Кафедра высокопроизводительных вычислений
Russian Journal of Genetics
Q4
Q4


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