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The novel extremely psychrophilic luciferase from Metridia longa: Properties of a high-purity protein produced in insect cells
Автор | Marina D. Larionova | |
Автор | Svetlana V. Markova | |
Автор | Eugene S. Vysotski | |
Дата внесения | 2018-02-07T07:24:25Z | |
Дата, когда ресурс стал доступен | 2018-02-07T07:24:25Z | |
Дата публикации | 2017-01 | |
Библиографическое описание | Marina D. Larionova. The novel extremely psychrophilic luciferase from Metridia longa: Properties of a high-purity protein produced in insect cells [Текст] / Marina D. Larionova, Svetlana V. Markova, Eugene S. Vysotski // Biochemical and Biophysical Research Communications. — 2017. — Т. 483 (№ 1). — С. 772-778 | |
ISSN | 0006291X | |
URI (для ссылок/цитирований) | http://www.sciencedirect.com/science/article/pii/S0006291X16321118 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/69561 | |
Описание | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. | |
Аннотация | The bright bioluminescence of copepod Metridia longa is conditioned by a small secreted coelenterazinedependent luciferase (MLuc). To date, three isoforms of MLuc differing in length, sequences, and some properties were cloned and successfully applied as high sensitive bioluminescent reporters. In this work, we report cloning of a novel group of genes from M. longa encoding extremely psychrophilic isoforms of MLuc (MLuc2-type). The novel isoforms share only ~54e64% of protein sequence identity with the previously cloned isoforms and, consequently, are the product of a separate group of paralogous genes. The MLuc2 isoform with consensus sequence was produced as a natively folded protein using baculovirus/ insect cell expression system, purified, and characterized. The MLuc2 displays a very high bioluminescent activity and high thermostability similar to those of the previously characterized M. longa luciferase isoform MLuc7. However, in contrast to MLuc7 revealing the highest activity at 12-17 C and 0.5 M NaCl, the bioluminescence optima of MLuc2 isoforms are at ~5 C and 1 M NaCl. The MLuc2 adaptation to cold is also accompanied by decrease of melting temperature and affinity to substrate suggesting a more conformational flexibility of a protein structure. The luciferase isoforms with different temperature optima may provide adaptability of the M. longa bioluminescence to the changes of water temperature during diurnal vertical migrations. | |
Тема | Bioluminescence | |
Тема | Coelenterazine | |
Тема | Bioluminescent reporter | |
Тема | Psychrophilic enzyme | |
Тема | Molecular adaptation | |
Название | The novel extremely psychrophilic luciferase from Metridia longa: Properties of a high-purity protein produced in insect cells | |
Тип | Journal Article | |
Тип | Published Journal Article | |
Страницы | 772-778 | |
ГРНТИ | 34.17.15 | |
Дата обновления | 2018-02-07T07:24:25Z | |
DOI | 10.1016/j.bbrc.2016.12.067 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Базовая кафедра биотехнологии | |
Журнал | Biochemical and Biophysical Research Communications | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q3 |