On the mechanism of biological activation by tritium
URI (for links/citations):http://elib.sfu-kras.ru/handle/2311/27994
Rozhko, T. V.
Badun, G. A.
Razzhivina, I. A.
Guseynov, O. A.
Guseynova, V. E.
Kudryasheva, N. S.
Институт фундаментальной биологии и биотехнологии
Кафедра медицинской биологии
Кафедра водных и наземных экосистем
Journal Name:Journal of Environmental Radioactivity
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q2
Bibliographic Citation:Rozhko, T. V. On the mechanism of biological activation by tritium [Текст] / T. V. Rozhko, G. A. Badun, I. A. Razzhivina, O. A. Guseynov, V. E. Guseynova, N. S. Kudryasheva // Journal of Environmental Radioactivity. — 2016. — Т. 157. — С. 131-135
The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 2.36 MBq/cm2). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescent kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a “non-genomic” mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media.