Chromatographic study of the component composition and antioxidant activity of polyphenol complex in biological raw materials

Abstract

Recently, the attention of many researchers has been attracted by raw materials that contain flavonoids. The purpose of the research is to summarize the results of studies of the composition of flavonoids of a non-fat sea buckthorn meal, which is a secondary raw material for the production of sea buckthorn oil. In this case, the sum of the five identified flavonoid compounds was isolated by extraction and subsequent adsorption column chromatography. At this stage, the actual problem of identifying and determining individual isolated flavonoids was solved using chromatographic (TLC, HPLC) and spectroscopic methods in comparison with reliably known samples. During the study, the flavonoids were identified: isorhamnetin, kaempferol, myritin, quercetin and its glycoside rutin, quercetin and isorhamnetin predominate. Ultimately, the effective parameters of the method for the improvement of the competitiveness of dietary supplements are established.