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Synthesis of PHAs by Hydrogen Bacteria in a Pilot Production Process
Автор | Tatiana, Volova | |
Автор | Evgeniy, Kiselev | |
Автор | Natalia, Zhila | |
Автор | Ekaterina, Shishatskaya | |
Дата внесения | 2020-01-20T07:56:54Z | |
Дата, когда ресурс стал доступен | 2020-01-20T07:56:54Z | |
Дата публикации | 2019-09 | |
Библиографическое описание | Tatiana, Volova. Synthesis of PHAs by Hydrogen Bacteria in a Pilot Production Process [Текст] / Volova Tatiana, Kiselev Evgeniy, Zhila Natalia, Shishatskaya Ekaterina // Biomacromolecules. — 2019. — Т. 20 (№ 9). — С. 3261-3270 | |
ISSN | 15257797 | |
URI (для ссылок/цитирований) | https://pubs.acs.org/doi/10.1021/acs.biomac.9b00295 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/129296 | |
Описание | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. | |
Аннотация | Synthesis of polyhydroxyalkanoates (PHA) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus eutrophus B-10646. Over 60 h of cultivation, cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in cell concentration of 130±10 g/L. Polymer content and yield coefficients for the biomass, kg biomass/kg carbon substrate, were similar on all substrates (80-85% and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Bipolymers poly(3-hydroxybutyrate/3-hydroxyvalerate) and poly(3-hydroxybutyrate/4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. eutrophus B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in organotrophic C. eutrophus B-10646 culture. | |
Тема | pilot production of PHA | |
Тема | Cupriavidus. eutrophus B-10646 | |
Тема | sugars | |
Тема | glycerol | |
Тема | composition and properties of PHA | |
Тема | carbon substrate consumption | |
Название | Synthesis of PHAs by Hydrogen Bacteria in a Pilot Production Process | |
Тип | Journal Article | |
Тип | Journal Article Preprint | |
Страницы | 3261-3270 | |
ГРНТИ | 62.13 | |
Дата обновления | 2020-01-20T07:56:54Z | |
DOI | 10.1021/acs.biomac.9b00295 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Базовая кафедра биотехнологии | |
Журнал | Biomacromolecules | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q1 |