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Kirill A. Lukyanenko
Ivan A. Denisov
Vladimir V. Sorokin
Anton S. Yakimov
Elena N. Esimbekova
Peter I. Belobrov
2020-01-20T07:56:31Z
2020-01-20T07:56:31Z
2019-03
Kirill A. Lukyanenko. Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples [Текст] / Kirill A. Lukyanenko, Ivan A. Denisov, Vladimir V. Sorokin, Anton S. Yakimov, Elena N. Esimbekova, Peter I. Belobrov // Chemosensors. — 2019. — Т. 7 (№ 1). — С. 16
https://www.mdpi.com/2227-9040/7/1/16
http://elib.sfu-kras.ru/handle/2311/129254
Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.
chemical measurements
silicon photomultiplier
optical biosensor
bioassay
microfluidics
luciferase
bioluminescence
Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples
Journal Article
Published Journal Article
16
29.31.23
2020-01-20T07:56:31Z
10.3390/chemosensors7010016
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Chemosensors
Q2
без квартиля


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