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Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples
Автор | Kirill A. Lukyanenko | |
Автор | Ivan A. Denisov | |
Автор | Vladimir V. Sorokin | |
Автор | Anton S. Yakimov | |
Автор | Elena N. Esimbekova | |
Автор | Peter I. Belobrov | |
Дата внесения | 2020-01-20T07:56:31Z | |
Дата, когда ресурс стал доступен | 2020-01-20T07:56:31Z | |
Дата публикации | 2019-03 | |
Библиографическое описание | Kirill A. Lukyanenko. Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples [Текст] / Kirill A. Lukyanenko, Ivan A. Denisov, Vladimir V. Sorokin, Anton S. Yakimov, Elena N. Esimbekova, Peter I. Belobrov // Chemosensors. — 2019. — Т. 7 (№ 1). — С. 16 | |
URI (для ссылок/цитирований) | https://www.mdpi.com/2227-9040/7/1/16 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/129254 | |
Аннотация | Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction. | |
Тема | chemical measurements | |
Тема | silicon photomultiplier | |
Тема | optical biosensor | |
Тема | bioassay | |
Тема | microfluidics | |
Тема | luciferase | |
Тема | bioluminescence | |
Название | Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples | |
Тип | Journal Article | |
Тип | Published Journal Article | |
Страницы | 16 | |
ГРНТИ | 29.31.23 | |
Дата обновления | 2020-01-20T07:56:31Z | |
DOI | 10.3390/chemosensors7010016 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Кафедра биофизики | |
Журнал | Chemosensors | |
Квартиль журнала в Scopus | Q2 | |
Квартиль журнала в Web of Science | без квартиля |