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Tatiana, Volova
Evgeniy, Kiselev
Natalia, Zhila
Ekaterina, Shishatskaya
2020-01-20T07:54:31Z
2020-01-20T07:54:31Z
2019-09
Tatiana, Volova. Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process [Текст] / Volova Tatiana, Kiselev Evgeniy, Zhila Natalia, Shishatskaya Ekaterina // Biomacromolecules. — 2019. — Т. 20 (№ 9). — С. 3261-3270
15257797
https://pubs.acs.org/doi/pdf/10.1021/acs.biomac.9b00295
http://elib.sfu-kras.ru/handle/2311/129072
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
Synthesis of polyhydroxyalkanoates (PHA) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus eutrophus B-10646. Over 60 h of cultivation, cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in cell concentration of 130±10 g/L. Polymer content and yield coefficients for the biomass, kg biomass/kg carbon substrate, were similar on all substrates (80-85% and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Bipolymers poly(3-hydroxybutyrate/3-hydroxyvalerate) and poly(3-hydroxybutyrate/4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. eutrophus B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in organotrophic C. eutrophus B-10646 culture.
pilot production of PHA
Cupriavidus. eutrophus B-10646
sugars
glycerol
composition and properties of PHA
carbon substrate consumption
Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process
Journal Article
Journal Article Preprint
3261-3270
34.05
2020-01-20T07:54:31Z
10.1021/acs.biomac.9b00295
Институт фундаментальной биологии и биотехнологии
Кафедра медицинской биологии
Базовая кафедра биотехнологии
Biomacromolecules
Q1
Q1


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