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Oleg S. Sutormin
Irina E. Sukovataya
Shubhra, Pande
Valentina A. Kratasyuk
2019-07-01T07:28:07Z
2019-07-01T07:28:07Z
2018-10
Oleg S. Sutormin. Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase [Текст] / Oleg S. Sutormin, Irina E. Sukovataya, Pande Shubhra, Valentina A. Kratasyuk // Molecular Catalysis. — 2018. — Т. 458. — С. 60-66
24688231
https://www.sciencedirect.com/science/article/pii/S2468823118303298?via%3Dihub
https://elib.sfu-kras.ru/handle/2311/111089
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
One of the current trends of the modern biology figures out cellular enzyme behaviour. Numerous researches look more closely at the chemical composition of creating in vivo simulated media conditions. The aim of this work was to find out a thermodynamic cooperativity of enzymes in a triple-enzyme chain (lactate dehydrogenase+NAD(P)H: FMN-oxidoreductase+bacterial luciferase) under in vivo simulated condition. The thermodynamic cooperativity effects were found out based on the influence of the viscogens (glycerol and sucrose) on the thermal stability of the triple-enzyme system. The results showed that the viscogens do not lead to an increase in the thermal stability of the triple-enzyme system. In addition, organic solvents (sucrose and glycerol) added as viscous agents to the reaction medium altered the kinetics of this triple-enzyme chain, including changing the light emission decay constant (kdec) and quantum yield of luminescence (Q). Plus, sucrose was found to be more efficient in limiting the flexibility of enzymes than glycerol. The high sensitivity of the triple-enzyme system to the viscogens may be connected with a fact that lactate dehydrogenase does not bound with couple enzyme system NAD(P)H: FMN-oxidoreductase+bacterial luciferase inside the real cell. Since this approach may be used as a method to understand the real connection between enzymes in cellular multi-enzyme metabolic chains inside the luminous bacteria cell.
Bioluminescence
Protein stability
In vivo simulated media
Coupling of enzymes
Metabolic chain
Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase
Journal Article
Published Journal Article
60-66
34.17.15
2019-07-01T07:28:07Z
10.1016/j.mcat.2018.08.012
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Molecular Catalysis
Q1
Q4


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