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Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase
Автор | Oleg S. Sutormin | |
Автор | Irina E. Sukovataya | |
Автор | Shubhra, Pande | |
Автор | Valentina A. Kratasyuk | |
Дата внесения | 2019-07-01T07:28:07Z | |
Дата, когда ресурс стал доступен | 2019-07-01T07:28:07Z | |
Дата публикации | 2018-10 | |
Библиографическое описание | Oleg S. Sutormin. Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase [Текст] / Oleg S. Sutormin, Irina E. Sukovataya, Pande Shubhra, Valentina A. Kratasyuk // Molecular Catalysis. — 2018. — Т. 458. — С. 60-66 | |
ISSN | 24688231 | |
URI (для ссылок/цитирований) | https://www.sciencedirect.com/science/article/pii/S2468823118303298?via%3Dihub | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/111089 | |
Описание | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. | |
Аннотация | One of the current trends of the modern biology figures out cellular enzyme behaviour. Numerous researches look more closely at the chemical composition of creating in vivo simulated media conditions. The aim of this work was to find out a thermodynamic cooperativity of enzymes in a triple-enzyme chain (lactate dehydrogenase+NAD(P)H: FMN-oxidoreductase+bacterial luciferase) under in vivo simulated condition. The thermodynamic cooperativity effects were found out based on the influence of the viscogens (glycerol and sucrose) on the thermal stability of the triple-enzyme system. The results showed that the viscogens do not lead to an increase in the thermal stability of the triple-enzyme system. In addition, organic solvents (sucrose and glycerol) added as viscous agents to the reaction medium altered the kinetics of this triple-enzyme chain, including changing the light emission decay constant (kdec) and quantum yield of luminescence (Q). Plus, sucrose was found to be more efficient in limiting the flexibility of enzymes than glycerol. The high sensitivity of the triple-enzyme system to the viscogens may be connected with a fact that lactate dehydrogenase does not bound with couple enzyme system NAD(P)H: FMN-oxidoreductase+bacterial luciferase inside the real cell. Since this approach may be used as a method to understand the real connection between enzymes in cellular multi-enzyme metabolic chains inside the luminous bacteria cell. | |
Тема | Bioluminescence | |
Тема | Protein stability | |
Тема | In vivo simulated media | |
Тема | Coupling of enzymes | |
Тема | Metabolic chain | |
Название | Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase | |
Тип | Journal Article | |
Тип | Published Journal Article | |
Страницы | 60-66 | |
ГРНТИ | 34.17.15 | |
Дата обновления | 2019-07-01T07:28:07Z | |
DOI | 10.1016/j.mcat.2018.08.012 | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Кафедра биофизики | |
Журнал | Molecular Catalysis | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q4 |