Disposable luciferase‐based microfluidic chip for rapid assay of water pollution
URI (for links/citations):https://onlinelibrary.wiley.com/doi/abs/10.1002/bio.3508
Институт фундаментальной биологии и биотехнологии
Journal Quartile in Scopus:Q2
Journal Quartile in Web of Science:Q4
Bibliographic Citation:Ivan, Denisov. Disposable luciferase‐based microfluidic chip for rapid assay of water pollution [Текст] / Denisov Ivan, Lukyanenko Kirill, Yakimov Anton, Kukhtevich Igor, Esimbekova Elena, Петр Белобров // Luminescence. — 2018. — Т. 33 (№ 6). — С. 1054-1061
In the present study, we demonstrate the use of a disposable luciferase‐based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3‐dihydroxybenzene and 1,4‐benzoquinone for the proposed microfluidic biosensor measured 3 μM, 15 mM, and 2 μM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers.