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Лукьяненко, К. А.
Denisov, I. A.
Yakimov, A. S.
Esimbekova, E. N.
Belousov, K. I.
Bukatin, A. S.
Kukhtevich, I. V.
Sorokin, V. V.
Evstrapov, A. A.
Belobrov, P. I.
2019-07-01T07:27:39Z
2019-07-01T07:27:39Z
2017-12
Лукьяненко, К. А. Analytical Enzymatic Reactions in Microfluidic Chips [Текст] / К. А. Лукьяненко, I. A. Denisov, A. S. Yakimov, E. N. Esimbekova, K. I. Belousov, A. S. Bukatin, I. V. Kukhtevich, V. V. Sorokin, A. A. Evstrapov, P. I. Belobrov // Applied Biochemistry and Microbiology. — 2017. — Т. 53 (№ 7). — С. 775-780
00036838
https://link.springer.com/article/10.1134/S0003683817070043
http://elib.sfu-kras.ru/handle/2311/110999
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction involving NAD(P)H:FMN-oxidoreductase and luciferase. Measurement of the catalytic activity of these enzymes (under the influence of pollutants) is the basis of enzymatic bioassay of various liquids. It was found that all of the components of the reaction must be placed in the same cell of the chip to improve the reproducibility of the measurements. The use of starch gel as a carrier for immobilization and gelatin as a scaffold in the reactor of the chip enables the preservation of enzyme activity in the course of sealing the chip at room temperature. It is shown that the components of the reaction should be vigorously stirred in a microfluidic chip reactor to improve the efficiency of the analysis. As a result of the studies, a prototype of microfluidic chip based on the enzymatic bioluminescent reaction is proposed. It is characterized by a detection limit of copper sulfate of 3 μM that corresponds to the sensitivity of traditional lux-biosensors based on living cells. The analysis time is reduced to 1 min, and the analysis can be performed by individuals without special laboratory skills.
bioluminescence
luciferase
microfluidics
microfluidic chip
enzymatic bioassay
Analytical Enzymatic Reactions in Microfluidic Chips
Journal Article
Journal Article Preprint
775-780
34.17
2019-07-01T07:27:39Z
10.1134/S0003683817070043
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Applied Biochemistry and Microbiology
Q3
Q4


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