Nonspecific stress response to temperature increase in Gammarus lacustris Sars with respect to oxygen-limited thermal tolerance concept
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DOI:
10.7717/peerj.5571URI (для ссылок/цитирований):
https://peerj.com/articles/5571/https://elib.sfu-kras.ru/handle/2311/110663
Автор:
Kseniya, Vereshchagina
Elizaveta, Kondrateva
Denis, Axenov-Gribanov
Zhanna, Shatilina
Andrey, Khomich
Daria, Bedulina
Задереев, Егор
Maxim, Timofeyev
Коллективный автор:
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Дата:
2018-09Журнал:
PeerJКвартиль журнала в Scopus:
Q1Квартиль журнала в Web of Science:
Q2Библиографическое описание:
Kseniya, Vereshchagina. Nonspecific stress response to temperature increase in Gammarus lacustris Sars with respect to oxygen-limited thermal tolerance concept [Текст] / Vereshchagina Kseniya, Kondrateva Elizaveta, Axenov-Gribanov Denis, Shatilina Zhanna, Khomich Andrey, Bedulina Daria, Егор Задереев, Timofeyev Maxim // PeerJ. — 2018.Аннотация:
The previously undescribed dynamics of the heat shock protein HSP70 and subsequent lipid peroxidation products have been assessed alongside lactate dehydrogenase
activity for Gammarus lacustris Sars, an amphipod species from the saltwater Lake Shira (Republic of Khakassia). Individuals were exposed to a gradual temperature
increase of 1 ◦C/hour (total exposure duration of 26 hours) starting from the mean annual temperature of their habitat (7 ◦C) up to 33 ◦C. A complex of biochemical
reactions occurred when saltwater G. lactustris was exposed to the gradual changes in temperature. This was characterized by a decrease in lactate dehydrogenase activity
and the launching of lipid peroxidation. The HSP70 level did not change significantly during the entire experiment. In agreement with the concept of oxygen-limited thermal
tolerance, an accumulation of the most toxic lipid peroxides (triene conjugates and Schiff bases) in phospholipids occurred at the same time and temperature as the
accumulation of lactate. The main criterion overriding the temperature threshold was, therefore, the transition to anaerobiosis, confirmed by the elevated lactate levels as
observed in our previous associated study, and by the development of cellular stress, which was expressed by an accumulation of lipid peroxidation products. An earlier
hypothesis, based on freshwater individuals of the same species, has been confirmed whereby the increased thermotolerance ofG. lacustrisfrom the saltwater lake was caused
by differences in energy metabolism and energy supply of nonspecific cellular stressresponse mechanisms. With the development of global climate change, these reactions
could be advantageous for saltwater G. lacustris. The studied biochemical reactions can be used as biomarkers for the stress status of aquatic organisms when their habitat
temperature changes.