The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation

Krasitskaya, V. V.; Goncharova, N. S.; Biriukov, V. V.; Bashmakova, E. E.; Kabilov, M. R.; Baykov, I. K.; Sokolov, A. E.; Frank, L. A.

doi:10.1111/php.13274

Author:

Krasitskaya, V. V.

Goncharova, N. S.

Biriukov, V. V.

Bashmakova, E. E.

Kabilov, M. R.

Baykov, I. K.

Sokolov, A. E.

Frank, L. A.

Corporate Contributor:

Институт фундаментальной биологии и биотехнологии

Институт инженерной физики и радиоэлектроники

Базовая кафедра биотехнологии

Лаборатория лесной геномики

Кафедра экспериментальной физики и инновационных технологий

Date:

2020

Journal Name:

Photochemistry and Photobiology

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Bibliographic Citation:

Krasitskaya, V. V. The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation [Текст] / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov, E. E. Bashmakova, M. R. Kabilov, I. K. Baykov, A. E. Sokolov, L. A. Frank // Photochemistry and Photobiology. — 2020.

Abstract:

Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer–target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.

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