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Ivan, Denisov
Kirill, Lukyanenko
Anton, Yakimov
Igor, Kukhtevich
Elena, Esimbekova
Белобров, Петр
2019-07-01T07:27:39Z
2019-07-01T07:27:39Z
2018
Ivan, Denisov. Disposable luciferase‐based microfluidic chip for rapid assay of water pollution [Текст] / Denisov Ivan, Lukyanenko Kirill, Yakimov Anton, Kukhtevich Igor, Esimbekova Elena, Петр Белобров // Luminescence. — 2018. — Т. 33 (№ 6). — С. 1054-1061
15227235
https://onlinelibrary.wiley.com/doi/abs/10.1002/bio.3508
https://elib.sfu-kras.ru/handle/2311/111001
In the present study, we demonstrate the use of a disposable luciferase‐based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3‐dihydroxybenzene and 1,4‐benzoquinone for the proposed microfluidic biosensor measured 3 μM, 15 mM, and 2 μM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers.
bioassay
lab‐on‐a‐chip
luciferase
microfluidics
solvent bonding
Disposable luciferase‐based microfluidic chip for rapid assay of water pollution
Journal Article
Journal Article Preprint
1054-1061
34.17
2019-07-01T07:27:39Z
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Luminescence
Q2
Q4


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