Measurement of Ion Association Constants from Decrease of Peak Areas by Capillary Electrophoresis Technique with Indirect Spectrophotometric Detection

Abstract

The new method to determine association constants using capillary electrophoresis technique with indirect spectrophotometric detection was investigated. An association constant was calculated from decrease of corrected peak area. The equations for correction of peak areas and calculation of association constants were examined using zone mathematical model of electrophoretic ion motion. This method was used to measure association constant of calcium - ions with sulfate - ions: lg Kass (СаSO4) = 2,39±0,09.