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Денисов, Иван Андреевич
2018-02-07T07:29:08Z
2018-02-07T07:29:08Z
2017-04
Денисов, Иван Андреевич. Luciferase-based bioassay for rapid pollutants detection and classification by means of multilayer artificial neural networks [Текст] / Иван Андреевич Денисов // Sensors and Actuators, B: Chemical. — 2017. — Т. 242. — С. 653-657
09254005
http://www.sciencedirect.com/science/article/pii/S092540051631869X
https://elib.sfu-kras.ru/handle/2311/69863
Biosensors for rapid environmental pollution detection can be designed with biomodule based on the bacterial bioluminescent system. Usually this method returns total value of toxicity and does not allow to distinguish pollutants types. Herein we demonstrate the classification of pollutants by the kinetic analysis utilizing artificial neural networks with multilayer perceptron architecture. The kinetics of light emission of NAD(P)H:FMN-oxidoreductase-luciferase bioluminescent reaction was measured for clean water and in the presence of three environment pollutants (1,4-benzoquinone, copper sulfate and 1,3-dihydroxybenzene) separately with various concentrations. The efficiency of using multilayer perceptron with sigmoid activation function for processing of kinetics of light emission was estimated. It was shown that multilayer perceptrons allowing to distinguish pollutant class and concentration after sufficient training. The architecture consisted of 61 inputs neurons, 3 hidden layers and 3 output neurons was found optimum in sense of learning time for classification of three pollutants. Usage of simplest activation function sigmoid and backpropagation method for multilayer perceptron teaching providing the results been useful for smart signal processing in computational modules of biosensors.
Bioluminescence
Luciferase
Bioassay
Artificial neural networks
Perceptron
Machine learning
Luciferase-based bioassay for rapid pollutants detection and classification by means of multilayer artificial neural networks
Journal Article
Journal Article Preprint
653-657
34.17
2018-02-07T07:29:08Z
10.1016/j.snb.2016.11.071
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Sensors and Actuators, B: Chemical
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