Is bacterial luminescence response to low-dose radiation associated with mutagenicity?
Rozhko, T. V.
Guseynov, O. A.
Guseynova, V. E.
Devyatlovskaya, A. N.
Kudryasheva, N. S.
Институт фундаментальной биологии и биотехнологии
Кафедра медицинской биологии
Кафедра водных и наземных экосистем
Journal Name:Journal of Environmental Radioactivity
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q2
Bibliographic Citation:Rozhko, T. V. Is bacterial luminescence response to low-dose radiation associated with mutagenicity? [Текст] / T. V. Rozhko, O. A. Guseynov, V. E. Guseynova, Bondar, A. N. Devyatlovskaya, N. S. Kudryasheva // Journal of Environmental Radioactivity. — 2017. — Т. 177. — С. 261-265
Luminous marine bacteria are widely used in bioassays with luminescence intensity being a physiological parameter tested. The purpose of the study was to determine whether bacterial genetic alteration is responsible for bioluminescence kinetics change under low-dose radiation exposure. The alphaemitting radionuclide 241Am and beta-emitting radionuclide 3 H were used as the sources of low-dose ionizing radiation. Changes of bioluminescence kinetics of Photobacterium phosphoreum in solutions of 241Am(NO3)3, 7 kBq/L, and tritiated water, 100 MBq/L, were studied; bioluminescence kinetics stages (absence of effect, activation, and inhibition) were determined. Bacterial suspension was sampled at different stages of the bioluminescent kinetics; the doses accumulated by the samples were close or a little higher than a tentative limit of a low-dose interval: 0.10 and 0.85 Gy for 241Am, or 0.11 and 0.18 Gy for 3H. Sequence analysis of the 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose alpha and beta radiation in the bacterial samples. Previous results on bacterial DNA exposed to low-dose gamma radiation (0.25 Gy) were analyzed and compared to those for alpha and beta irradiation. It is concluded that bioluminescence activation and/or inhibition under the applied conditions of low-dose alpha, beta and gamma radioactive exposure is not associated with DNA mutations in the gene sequences tested