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Volova, Tatiana G.en
Kalacheva, Galina S.en
Steinbüchel, Alexanderen
2008-02-04T08:51:14Z
2008-02-04T08:51:14Z
2008-01
1997-1389
https://elib.sfu-kras.ru/handle/2311/638
The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild-type strains (H16 and B5786) it has been first found that under mixotrophic growth conditions – CO2 + co-substrate (alkanoic acids) – bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate, and hydroxyhexanoate as major monomers and hydroxyhexanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs has been synthesized and their physicochemical properties examined.en
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Сибирский федеральный университет. Siberian Federal Universityen
2008 (1)en
Журнал Сибирского федерального университета. Биология. Journal of Siberian Federal University. Biology
Wautersia eutropha, autotrophic and mixotrophic growth, multi-component polyhydroxyalkanoatesen
Biosynthesis of Multi-component Polyhydroxyalkanoates by the Bacterium Wautersia Eutrophaen
Journal Article
Published Journal Article
Volova, Tatiana G.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia; Siberian Federal University, Svobodny av. 79, Krasnoyarsk, 660041, Russiaen
Kalacheva, Galina S.: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia; kalach@ibp.ruen
Steinbüche, Alexander :Institute of Molecular Microbiology and Biotechnology, Westfalische Wilhelms-Universitat Munster, Corrensstrasse 3, D-48149 Germanyen
91-101
https://elib.krasu.ru/bitstream/2311/638/1/8_Volova.pdf


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