Ultraviolet ﬂuorescence of coelenteramide and coelenteramide-containing ﬂuorescent proteins. Experimental and theoretical study
URI (для ссылок/цитирований):http://elib.sfu-kras.ru/handle/2311/33250
Alieva, R. R.
Tomilin, F. N.
Kuzubov, A. A.
Ovchinnikov, S. G.
Kudryasheva, N. S.
Институт цветных металлов и материаловедения
Кафедра физической и неорганической химии
Журнал:Journal of Photochemistry & Photobiology, B: Biology
Квартиль журнала в Scopus:Q2
Библиографическое описание:Alieva, R. R. Ultraviolet ﬂuorescence of coelenteramide and coelenteramide-containing ﬂuorescent proteins. Experimental and theoretical study [Текст] / R. R. Alieva, F. N. Tomilin, A. A. Kuzubov, S. G. Ovchinnikov, N. S. Kudryasheva // Journal of Photochemistry & Photobiology, B: Biology. — 2016. — Т. 162. — С. 318-323
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
Coelenteramide-containing ﬂuorescent proteins are products of bioluminescent reactions of marine coelenter- ates. They are called ‘discharged photoproteins’. Their light-induced ﬂuorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-induced ﬂuorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexci- tation to the upper electron-excited states (260–300 nm) of the discharged photoproteins initiates a ﬂuorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UV ﬂuorescence, the light- induced ﬂuorescence spectra of coelenteramide (CLM), ﬂuorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330 nm) in the near UV region was observed in CLM ﬂuorescence at higher excitation energy (260–300 nm). Quantum chemical calculations by time depending method with B3LYP/cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UV ﬂuorescence peak. Quantum yields of CLM ﬂuorescence in methanol were 0.028 ± 0.005 at 270–340 nm pho- toexcitation. A conclusion was made that the UV emission of CLM might contribute to the UV ﬂuorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures – complexes of proteins with low-weight aromatic molecules.