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Alieva, R. R.
Tomilin, F. N.
Kuzubov, A. A.
Ovchinnikov, S. G.
Kudryasheva, N. S.
2017-06-16T10:28:04Z
2017-06-16T10:28:04Z
2016-09
Alieva, R. R. Ultravioletfluorescence of coelenteramide and coelenteramide-containingfluorescent proteins. Experimental and theoretical study [Текст] / R. R. Alieva, F. N. Tomilin, A. A. Kuzubov, S. G. Ovchinnikov, N. S. Kudryasheva // Journal of Photochemistry and Photobiology B: Biology. — 2016. — № 162. — С. 318-323
10111344
http://elib.sfu-kras.ru/handle/2311/33063
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
Coelenteramide-containingfluorescent proteins are products of bioluminescent reactions of marine coelenter-ates. They re called‘discharged photoproteins’. Their light-inducedfluorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-inducedfluorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexci-tation to the upper electron-excited states (260–300 nm) of the discharged photoproteins initiates afluorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UVfluorescence, the light-inducedfluorescence spectra of coelenteramide (CLM),fluorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330 nm) in the near UV region was observed in CLMfluorescence at higher excitation energy (260–300 nm). Quantum chemical calculations by time depending method with B3LYP/cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UVfluorescence peak. Quantum yields of CLMfluorescence in methanol were 0.028 ± 0.005 at 270–340 nm pho-toexcitation. A conclusion was made that the UV emission of CLM might contribute to the UVfluorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures – complexes of proteins with low-weight aromatic molecules.
http://www.sciencedirect.com/science/article/pii/S1011134416304262?np=y
Coelenteramide
Fluorescent protein
Discharged photoproteins
Obelin
Aequorin
Fluorescence
Excitation energy
B3LYP
Ultravioletfluorescence of coelenteramide and coelenteramide-containingfluorescent proteins. Experimental and theoretical study
Journal Article
Published Journal Article
318-323
34.17.09
2017-06-16T10:28:04Z
10.1016/j.jphotobiol.2016.07.004
Институт цветных металлов и материаловедения
Кафедра аналитической и органической химии
Journal of Photochemistry and Photobiology B: Biology
Q2
Q2


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