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Ultravioletfluorescence of coelenteramide and coelenteramide-containingfluorescent proteins. Experimental and theoretical study
Автор | Alieva, R. R. | |
Автор | Tomilin, F. N. | |
Автор | Kuzubov, A. A. | |
Автор | Ovchinnikov, S. G. | |
Автор | Kudryasheva, N. S. | |
Дата внесения | 2017-06-16T10:28:04Z | |
Дата, когда ресурс стал доступен | 2017-06-16T10:28:04Z | |
Дата публикации | 2016-09 | |
Библиографическое описание | Alieva, R. R. Ultravioletfluorescence of coelenteramide and coelenteramide-containingfluorescent proteins. Experimental and theoretical study [Текст] / R. R. Alieva, F. N. Tomilin, A. A. Kuzubov, S. G. Ovchinnikov, N. S. Kudryasheva // Journal of Photochemistry and Photobiology B: Biology. — 2016. — № 162. — С. 318-323 | |
ISSN | 10111344 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/33063 | |
Описание | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. | |
Аннотация | Coelenteramide-containingfluorescent proteins are products of bioluminescent reactions of marine coelenter-ates. They re called‘discharged photoproteins’. Their light-inducedfluorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-inducedfluorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexci-tation to the upper electron-excited states (260–300 nm) of the discharged photoproteins initiates afluorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UVfluorescence, the light-inducedfluorescence spectra of coelenteramide (CLM),fluorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330 nm) in the near UV region was observed in CLMfluorescence at higher excitation energy (260–300 nm). Quantum chemical calculations by time depending method with B3LYP/cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UVfluorescence peak. Quantum yields of CLMfluorescence in methanol were 0.028 ± 0.005 at 270–340 nm pho-toexcitation. A conclusion was made that the UV emission of CLM might contribute to the UVfluorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures – complexes of proteins with low-weight aromatic molecules. | |
Ссылка на другой сайт | http://www.sciencedirect.com/science/article/pii/S1011134416304262?np=y | |
Тема | Coelenteramide | |
Тема | Fluorescent protein | |
Тема | Discharged photoproteins | |
Тема | Obelin | |
Тема | Aequorin | |
Тема | Fluorescence | |
Тема | Excitation energy | |
Тема | B3LYP | |
Название | Ultravioletfluorescence of coelenteramide and coelenteramide-containingfluorescent proteins. Experimental and theoretical study | |
Тип | Journal Article | |
Тип | Published Journal Article | |
Страницы | 318-323 | |
ГРНТИ | 34.17.09 | |
Дата обновления | 2017-06-16T10:28:04Z | |
DOI | 10.1016/j.jphotobiol.2016.07.004 | |
Институт | Институт цветных металлов и материаловедения | |
Подразделение | Кафедра аналитической и органической химии | |
Журнал | Journal of Photochemistry and Photobiology B: Biology | |
Квартиль журнала в Scopus | Q2 | |
Квартиль журнала в Web of Science | Q2 |