The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation
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DOI:
10.1111/php.13274URI (для ссылок/цитирований):
https://onlinelibrary.wiley.com/doi/abs/10.1111/php.13274https://elib.sfu-kras.ru/handle/2311/142375
Автор:
Krasitskaya, V. V.
Goncharova, N. S.
Biriukov, V. V.
Bashmakova, E. E.
Kabilov, M. R.
Baykov, I. K.
Sokolov, A. E.
Frank, L. A.
Коллективный автор:
Институт фундаментальной биологии и биотехнологии
Институт инженерной физики и радиоэлектроники
Базовая кафедра биотехнологии
Лаборатория лесной геномики
Кафедра экспериментальной физики и инновационных технологий
Дата:
2020Журнал:
Photochemistry and PhotobiologyКвартиль журнала в Scopus:
Q1Квартиль журнала в Web of Science:
Q2Библиографическое описание:
Krasitskaya, V. V. The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation [Текст] / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov, E. E. Bashmakova, M. R. Kabilov, I. K. Baykov, A. E. Sokolov, L. A. Frank // Photochemistry and Photobiology. — 2020.Аннотация:
Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer–target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent
assay.