Genetic variation and signatures of natural selection in populations of European beech (Fagus sylvatica L.) along precipitation gradients
URI (for links/citations):https://link.springer.com/article/10.1007%2Fs11295-018-1297-2
Konstantin V. Krutovsky
Институт фундаментальной биологии и биотехнологии
Базовая кафедра защиты и современных технологии мониторинга лесов
Journal Name:Tree Genetics & Genomes
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q1
Bibliographic Citation:Laura, Cuervo-Alarcon. Genetic variation and signatures of natural selection in populations of European beech (Fagus sylvatica L.) along precipitation gradients [Текст] / Cuervo-Alarcon Laura, Arend Matthias, Müller Markus, Sperisen Christoph, Finkeldey Reiner, Konstantin V. Krutovsky // Tree Genetics & Genomes. — 2018. — Т. 14 (№ 84).
European beech (Fagus sylvatica L.) is one of the most important forest tree species in Europe, and its genetic adaptation potential to climate change is of great interest. Saplings and adults from 12 European beech populations were sampled along two steep precipitation gradients in Switzerland. All individuals were genotyped at 13 microsatellite markers and 70 SNPs in 24 stress response and phenology related candidate genes. Both SSR and SNP markers had high genetic diversity in the studied populations and low but statistically significant population differentiation. Two approaches were used to discover SNPs with signatures of selection: search for FST SNP outliers and analyses of SNP associations with environmental variables such as temperatures, precipitation and humidity. Three (4.3%) SNPs were consistently identified as outliers in the adults by more than one method, and they were very likely under positive selection. Twenty (28.6%) SNPs in the saplings and 10 (14.3%) SNPs in the adults were associated with environmental variables found by more than one method. In general, there were 22 (31.4%) SNPs in 17 (70.8%) candidate genes in the saplings, and 16 (22.9%) SNPs in 10 (41.6%) candidate genes in the adults, consistently identified by at least two of the five methods used, indicating that they are very likely under selection. Genes with SNPs showing signatures of selection are involved in a wide range of molecular functions, such as oxidoreductases (IDH), hydrolases (CysPro), transferases (XTH), transporters (KT2), chaperones (CP10) and transcription factors (DAG, NAC transcription factor). The obtained data will help us better understand the genetic variation underlying adaptation to environmentally changing conditions in European beech, which is of great importance for the development of scientific guidelines for the sustainable management and conservation of this important species.