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Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma
Автор | Bashmakova, E. E. | |
Автор | Krasitskaya, V. V. | |
Автор | Zamay, G. S. | |
Автор | Zamay, T. N. | |
Автор | Frank, L. A. | |
Дата внесения | 2020-01-20T07:55:35Z | |
Дата, когда ресурс стал доступен | 2020-01-20T07:55:35Z | |
Дата публикации | 2019-07 | |
Библиографическое описание | Bashmakova, E. E. Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma [Текст] / E. E. Bashmakova, V. V. Krasitskaya, G. S. Zamay, T. N. Zamay, L. A. Frank // Talanta. — 2019. — Т. 199. — С. 674-678 | |
ISSN | 00399140 | |
URI (для ссылок/цитирований) | http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=D4iFjBThJ3uJIVejtux&page=1&doc=1 | |
URI (для ссылок/цитирований) | https://elib.sfu-kras.ru/handle/2311/129161 | |
Аннотация | Two high-affinity DNA aptamers for lung tumor cells were applied as biospecific elements in bioluminescent assay of patient blood. The oligonucleotide complementary to the 5' end of both aptamers carrying either biotin or Ca2+-regulated photoprotein obelin was used to form a sandwich-type analytical complex on the surfaces of magnetic streptavidin-activated microspherical particles. Clinical blood samples from cases of morphologically confirmed lung cancer and control samples were analyzed applying the developed assay. From the receiver operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers the sensitivity of 91.5% and the specificity of 75% (p<0.001). The area under ROC curve with the value of 0.901 distinguishes well between the two groups under investigation. | |
Тема | DNA aptamers | |
Тема | lung tumor | |
Тема | bioluminescent solid-phase microassay | |
Название | Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma | |
Тип | Journal Article | |
Тип | Journal Article Preprint | |
Страницы | 674-678 | |
ГРНТИ | 34.15.23 | |
Дата обновления | 2020-01-20T07:55:35Z | |
Институт | Институт фундаментальной биологии и биотехнологии | |
Подразделение | Базовая кафедра биотехнологии | |
Журнал | Talanta | |
Квартиль журнала в Scopus | Q1 | |
Квартиль журнала в Web of Science | Q1 |