Показать сокращенную информацию

Bashmakova, E. E.
Krasitskaya, V. V.
Zamay, G. S.
Zamay, T. N.
Frank, L. A.
2020-01-20T07:55:35Z
2020-01-20T07:55:35Z
2019-07
Bashmakova, E. E. Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma [Текст] / E. E. Bashmakova, V. V. Krasitskaya, G. S. Zamay, T. N. Zamay, L. A. Frank // Talanta. — 2019. — Т. 199. — С. 674-678
00399140
http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=D4iFjBThJ3uJIVejtux&page=1&doc=1
https://elib.sfu-kras.ru/handle/2311/129161
Two high-affinity DNA aptamers for lung tumor cells were applied as biospecific elements in bioluminescent assay of patient blood. The oligonucleotide complementary to the 5' end of both aptamers carrying either biotin or Ca2+-regulated photoprotein obelin was used to form a sandwich-type analytical complex on the surfaces of magnetic streptavidin-activated microspherical particles. Clinical blood samples from cases of morphologically confirmed lung cancer and control samples were analyzed applying the developed assay. From the receiver operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers the sensitivity of 91.5% and the specificity of 75% (p<0.001). The area under ROC curve with the value of 0.901 distinguishes well between the two groups under investigation.
DNA aptamers
lung tumor
bioluminescent solid-phase microassay
Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma
Journal Article
Journal Article Preprint
674-678
34.15.23
2020-01-20T07:55:35Z
Институт фундаментальной биологии и биотехнологии
Базовая кафедра биотехнологии
Talanta
Q1
Q1


Файлы в этом документе

Thumbnail

Данный элемент включен в следующие коллекции

Показать сокращенную информацию