Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)
URI (for links/citations):https://www.sciencedirect.com/science/article/pii/S014181301836851X
Volova, T. G.
Shumilova, A. A.
Nikolaeva, E. D.
Kirichenko, A. K.
Shishatskaya, E. I.
Институт фундаментальной биологии и биотехнологии
Кафедра медицинской биологии
Базовая кафедра биотехнологии
Journal Name:International Journal of Biological Macromolecules
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q1
Bibliographic Citation:Volova, T. G. Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB) [Текст] / T. G. Volova, A. A. Shumilova, E. D. Nikolaeva, A. K. Kirichenko, E. I. Shishatskaya // International Journal of Biological Macromolecules. — 2019. — Т. 131. — С. 230-240
Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing.