Fluorescence lifetime components reveal kinetic intermediate states upon equilibrium denaturation of carbonic anhydrase II
URI (for links/citations):http://iopscience.iop.org/article/10.1088/2050-6120/aa994a/meta
Немцева, Е. В.
Нагибина, Г. С.
Мельник, Богдан С.
Институт фундаментальной биологии и биотехнологии
Институт инженерной физики и радиоэлектроники
Кафедра общей физики
Journal Name:Methods and Applications in Fluorescence
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q2
Bibliographic Citation:Немцева, Е. В. Fluorescence lifetime components reveal kinetic intermediate states upon equilibrium denaturation of carbonic anhydrase II [Текст] / Е. В. Немцева, Олеся Лащук, Марина Герасимова, Татьяна Мельник, Г. С. Нагибина, Богдан С. Мельник // Methods and Applications in Fluorescence. — 2018. — Т. 6 (№ 1). — С. 1-9
In most cases, intermediate states of multistage folding proteins are not ‘visible’ under equilibrium conditions but are revealed in kinetic experiments. Time-resolved fluorescence spectroscopy was used in equilibrium denaturation studies. The technique allows for detecting changes in the conformation and environment of tryptophan residues in different structural elements of carbonic anhydrase II which in its turn has made it possible to study the intermediate states of carbonic anhydrase II under equilibrium conditions. The results of equilibrium and kinetic experiments using wild-type bovine carbonic anhydrase II and its mutant form with the substitution of leucine for alanine at position 139 (L139A) were compared. The obtained lifetime components of intrinsic tryptophan fluorescence allowed for revealing that, the same as in kinetic experiments, under equilibrium conditions the unfolding of carbonic anhydrase II ensues through formation of intermediate states.
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