FLUORESCENT PROTEIN-BASED BIOASSAY FOR LOW-DOSE GAMMA RADIATION
URI (for links/citations):https://link.springer.com/article/10.1007/s00216-018-1282-5
Petrova, A. S.
Lukonina, A. S.
Dementyev, D. V.
Bolsunovsky, A. Ya.
Popov, A. V.
Кудряшева, Н. С.
Институт фундаментальной биологии и биотехнологии
Journal Name:Analytical and bioanalytical Chemistry
Journal Quartile in Scopus:Q1
Journal Quartile in Web of Science:Q1
Bibliographic Citation:Petrova, A. S. FLUORESCENT PROTEIN-BASED BIOASSAY FOR LOW-DOSE GAMMA RADIATION [Текст] / A. S. Petrova, A. S. Lukonina, D. V. Dementyev, A. Ya. Bolsunovsky, A. V. Popov, Н. С. Кудряшева // Analytical and bioanalytical Chemistry. — 2018.
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.
The study develops an application of fluorescent coelenteramide-containing protein as a color bioassay for radiotoxicity evaluation. Biological effect of chronic low-dose gamma radiation was under investigation. The Cs-137 containing radioactive particle (2.07 mGy/h) was used as a source of low-intensive gamma radiation. ‘Discharged obelin’, product of bioluminescent reaction of marine coelenterate Obelia longissimi, was applied as a representative of the coelenteramide-containing protein group. Coelenteramide, fluorophore of discharged obelin, is a photochemically active molecule; it produces fluorescence forms of different color. Contributions of these forms (‘violet’ and ‘blue-green’) to the visible fluorescence depend on the efficiency of photochemical proton transfer in protein-bound coelenteramide; the contributions are controlled by coelenteramide’ microenvironment, and, hence, evaluate distractive ability and toxicity of gamma radiation. The protein samples were exposed to gamma radiation for 7 and 16 days at 20°C and 5°C respectively; maximal dose accumulated by the samples was 0.35 Gy being close to a tentative limit of a low-dose interval. As a result, two fluorescent characteristics (overall fluorescent intensity and contributions of color components to the fluorescent spectra) are suggested as bioassay parameters. Both parameters demonstrated high sensitivity of the protein-based bioassay to low-dose gamma radiation (to 0.03 -0.05 Gy). Higher temperature (20°C) enhanced the response of the protein to the radiation. The study develops physico-chemical understanding of radiotoxic effects.