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Лукьяненко, Кирилл А.
Kirill I. Belousov
Ivan A. Denisov
Anton S. Yakimov
Elena N. Esimbekova
Anton S. Bukatin
Anatoly A. Evstrapov
Peter I. Belobrov
2019-07-01T07:27:38Z
2019-07-01T07:27:38Z
2017
Лукьяненко, Кирилл А. Active mixing of immobilised enzymatic system in microfluidic chip [Текст] / Кирилл А. Лукьяненко, Kirill I. Belousov, Ivan A. Denisov, Anton S. Yakimov, Elena N. Esimbekova, Anton S. Bukatin, Anatoly A. Evstrapov, Peter I. Belobrov // Micro and Nano Letters. — 2017. — Т. 12 (№ 6). — С. 377-381
17500443
http://digital-library.theiet.org/content/journals/10.1049/mnl.2016.0646
https://elib.sfu-kras.ru/handle/2311/110997
Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electro-mechanical speaker's membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips.
molecular biophysics
biological techniques
numerical analysis
bioMEMS
biochemistry
enzymes
Active mixing of immobilised enzymatic system in microfluidic chip
Journal Article
Journal Article Preprint
377-381
34.17
2019-07-01T07:27:38Z
10.1049/mnl.2016.0646
Институт фундаментальной биологии и биотехнологии
Кафедра биофизики
Micro and Nano Letters
Q3


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